Load all required libraries.
library(tidyverse)
## -- Attaching packages --------------------------------------- tidyverse 1.3.1 --
## v ggplot2 3.3.5 v purrr 0.3.4
## v tibble 3.1.3 v dplyr 1.0.7
## v tidyr 1.1.3 v stringr 1.4.0
## v readr 2.0.0 v forcats 0.5.1
## -- Conflicts ------------------------------------------ tidyverse_conflicts() --
## x dplyr::filter() masks stats::filter()
## x dplyr::lag() masks stats::lag()
library(plotly)
##
## Attaching package: 'plotly'
## The following object is masked from 'package:ggplot2':
##
## last_plot
## The following object is masked from 'package:stats':
##
## filter
## The following object is masked from 'package:graphics':
##
## layout
library(broom)
Read in raw data from RDS.
raw_data <- readRDS("./year2.RDS")
Make a few small modifications to names and data for visualizations.
final_data <- raw_data %>% mutate(log_copy_per_L = log10(mean_copy_num_L)) %>%
rename(Facility = wrf) %>%
mutate(Facility = recode(Facility,
"NO" = "WRF A",
"MI" = "WRF B",
"CC" = "WRF C"))
Seperate the data by gene target to ease layering in the final plot
#make three data layers
only_positives <<- subset(final_data, (!is.na(final_data$Facility)))
only_n1 <- subset(only_positives, target == "N1")
only_n2 <- subset(only_positives, target == "N2")
only_background <<-final_data %>%
select(c(date, cases_cum_clarke, new_cases_clarke, X7_day_ave_clarke)) %>%
group_by(date) %>% summarise_if(is.numeric, mean)
#specify fun colors
background_color <- "#7570B3"
seven_day_ave_color <- "#E6AB02"
marker_colors <- c("N1" = '#1B9E77',"N2" ='#D95F02')
#remove facilty C for now
#only_n1 <- only_n1[!(only_n1$Facility == "WRF C"),]
#only_n2 <- only_n2[!(only_n2$Facility == "WRF C"),]
only_n1 <- only_n1[!(only_n1$Facility == "WRF A" & only_n1$date == "2020-11-02"), ]
only_n2 <- only_n2[!(only_n2$Facility == "WRF A" & only_n2$date == "2020-11-02"), ]
Build the main plot
#first layer is the background epidemic curve
p1 <- only_background %>%
plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~new_cases_clarke,
type = "bar",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Daily Cases: ', new_cases_clarke),
alpha = 0.5,
name = "Daily Reported Cases",
color = background_color,
colors = background_color,
showlegend = FALSE) %>%
layout(yaxis = list(title = "Clarke County Daily Cases", showline=TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#renders the main plot layer two as seven day moving average
p1 <- p1 %>% plotly::add_trace(x = ~date, y = ~X7_day_ave_clarke,
type = "scatter",
mode = "lines",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Seven-Day Moving Average: ', X7_day_ave_clarke),
name = "Seven Day Moving Average Athens",
line = list(color = seven_day_ave_color),
showlegend = FALSE)
#renders the main plot layer three as positive target hits
p2 <- plotly::plot_ly() %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n1,
symbol = ~Facility,
marker = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
type = "scatter",
mode = "markers",
hoverinfo = "text",
text = ~paste('</br> Date: ', date,
'</br> Facility: ', Facility,
'</br> Target: ', target,
'</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
data = only_n2,
symbol = ~Facility,
marker = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(yaxis = list(title = "SARS CoV-2 Copies/L",
showline = TRUE,
type = "log",
dtick = 1,
automargin = TRUE)) %>%
layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
#adds the limit of detection dashed line
p2 <- p2 %>% plotly::add_segments(x = as.Date("2021-06-30"),
xend = ~max(date + 10),
y = 3571.429, yend = 3571.429,
opacity = 0.35,
line = list(color = "black", dash = "dash")) %>%
layout(annotations = list(x = as.Date("2021-06-30"), y = 3.8, xref = "x", yref = "y",
text = "Limit of Detection", showarrow = FALSE))
p1
p2
Combine the two main plot pieces as a subplot
#seperate n1 and n2 frames by site
#n1
wrf_a_only_n1 <- subset(only_n1, Facility == "WRF A")
wrf_b_only_n1 <- subset(only_n1, Facility == "WRF B")
wrf_c_only_n1 <- subset(only_n1, Facility == "WRF C")
#n2
wrf_a_only_n2 <- subset(only_n2, Facility == "WRF A")
wrf_b_only_n2 <- subset(only_n2, Facility == "WRF B")
wrf_c_only_n2 <- subset(only_n2, Facility == "WRF C")
#rejoin the old data frames then seperate in to averages for each plant.
wrfa_both <- full_join(wrf_a_only_n1, wrf_a_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
wrfb_both <- full_join(wrf_b_only_n1, wrf_b_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
wrfc_both <- full_join(wrf_c_only_n1, wrf_c_only_n2)%>%
select(c(date, mean_total_copies)) %>%
group_by(date) %>%
summarize_if(is.numeric, mean) %>%
ungroup() %>%
mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
#get max date
maxdate <- max(wrfa_both$date)
mindate <- min(wrfa_both$date)
Build loess smoothing figures figures
This makes the individual plots
#**************************************WRF A PLOT**********************************************
#add trendlines
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_botha <- ggplot(wrfa_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_botha<<-..y..), method = "loess", color = '#1B9E77',
span = 0.3, n = 127)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_botha
## `geom_smooth()` using formula 'y ~ x'
fit_botha
## [1] 11.56390 11.59195 11.62090 11.65071 11.68132 11.71269 11.74476 11.77776
## [9] 11.81180 11.84666 11.88216 11.91809 11.95426 11.99234 12.03330 12.07583
## [17] 12.11864 12.16045 12.19997 12.23590 12.26763 12.29622 12.32290 12.34889
## [25] 12.37543 12.40373 12.43504 12.46950 12.50571 12.54251 12.57877 12.61333
## [33] 12.64505 12.67279 12.69456 12.71050 12.72260 12.73289 12.74339 12.75610
## [41] 12.77303 12.77551 12.77996 12.80925 12.84769 12.89217 12.93957 12.98677
## [49] 13.03065 13.06811 13.10335 13.14107 13.17883 13.21420 13.24473 13.26800
## [57] 13.28156 13.28255 13.27182 13.25252 13.22782 13.20086 13.17481 13.15283
## [65] 13.13432 13.11631 13.09841 13.08021 13.06130 13.04129 13.01977 12.99812
## [73] 12.97690 12.95445 12.92914 12.89932 12.86477 12.82684 12.78631 12.74393
## [81] 12.70047 12.65668 12.61332 12.56743 12.51729 12.46559 12.41499 12.36816
## [89] 12.32777 12.29650 12.27567 12.26297 12.25562 12.25083 12.24584 12.23785
## [97] 12.22408 12.20372 12.17978 12.15523 12.13305 12.11621 12.10769 12.11047
## [105] 12.14566 12.18063 12.19320 12.21114 12.23162 12.25184 12.26897 12.28020
## [113] 12.28271 12.27761 12.26850 12.25628 12.24189 12.22621 12.21018 12.19469
## [121] 12.17870 12.16080 12.14136 12.12075 12.09932 12.07744 12.05549
#assign fits to a vector
both_trenda <- fit_botha
#extract y min and max for each
limits_botha <- ggplot_build(extract_botha)$data
## `geom_smooth()` using formula 'y ~ x'
limits_botha <- as.data.frame(limits_botha)
both_ymina <- limits_botha$ymin
both_ymaxa <- limits_botha$ymax
#reassign dataframes (just to be safe)
work_botha <- wrfa_both
#fill in missing dates to smooth fits
work_botha <- work_botha %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_botha <- work_botha$date
#create a new smooth dataframe to layer
smooth_frame_botha <- data.frame(date_vec_botha, both_trenda, both_ymina, both_ymaxa)
#WRF A
#plot smooth frames
p_wrf_a <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_botha, y = ~both_trenda,
data = smooth_frame_botha,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_botha,
'</br> Median Log Copies: ', round(both_trenda, digits = 2)),
line = list(color = '#1B9E77', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_botha, ymin = ~both_ymina, ymax = ~both_ymaxa,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_botha, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxa, digits = 2),
'</br> Min Log Copies: ', round(both_ymina, digits = 2)),
name = "",
fillcolor = '#1B9E77',
line = list(color = '#1B9E77')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF A") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfa_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#1B9E77', size = 6, opacity = 0.65))
p_wrf_a
save(p_wrf_a, file = "./site_objects/wrf_a_year2.rda")
#**************************************WRF B PLOT**********************************************
#add trendlines
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_bothb <- ggplot(wrfb_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_bothb<<-..y..), method = "loess", color = '#D95F02',
span = 0.3, n = 127)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothb
## `geom_smooth()` using formula 'y ~ x'
fit_bothb
## [1] 10.70603 10.80235 10.89577 10.98636 11.07413 11.15912 11.24137 11.32021
## [9] 11.39542 11.46772 11.53780 11.60640 11.67422 11.73825 11.79628 11.85025
## [17] 11.90208 11.95370 12.00705 12.06405 12.12926 12.20352 12.28326 12.36487
## [25] 12.44477 12.51935 12.58504 12.64277 12.69556 12.74291 12.78430 12.81924
## [33] 12.84722 12.86773 12.87775 12.87693 12.86876 12.85675 12.84438 12.83516
## [41] 12.83257 12.81863 12.80626 12.81781 12.83996 12.86914 12.90177 12.93429
## [49] 12.96311 12.98467 13.00029 13.01369 13.02490 13.03395 13.04087 13.04569
## [57] 13.04843 13.04677 13.03931 13.02749 13.01272 12.99643 12.98005 12.96500
## [65] 12.94984 12.93275 12.91460 12.89629 12.87867 12.86263 12.84905 12.83342
## [73] 12.81371 12.79451 12.78041 12.77599 12.78451 12.80342 12.82860 12.85589
## [81] 12.88116 12.90028 12.90910 12.91203 12.91492 12.91582 12.91274 12.90374
## [89] 12.88684 12.86009 12.82051 12.76932 12.71086 12.64948 12.58950 12.53526
## [97] 12.49112 12.45244 12.41277 12.37364 12.33660 12.30318 12.27493 12.25340
## [105] 12.24080 12.23216 12.23369 12.25286 12.28229 12.31460 12.34239 12.35829
## [113] 12.35491 12.33564 12.30817 12.27150 12.22465 12.16666 12.09653 12.01328
## [121] 11.91790 11.81182 11.69479 11.56656 11.42687 11.27546 11.11208
#assign fits to a vector
both_trendb <- fit_bothb
#extract y min and max for each
limits_bothb <- ggplot_build(extract_bothb)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothb <- as.data.frame(limits_bothb)
both_yminb <- limits_bothb$ymin
both_ymaxb <- limits_bothb$ymax
#reassign dataframes (just to be safe)
work_bothb <- wrfb_both
#fill in missing dates to smooth fits
work_bothb <- work_bothb %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothb <- work_bothb$date
#create a new smooth dataframe to layer
smooth_frame_bothb <- data.frame(date_vec_bothb, both_trendb, both_yminb, both_ymaxb)
#WRF B
#plot smooth frames
p_wrf_b <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_bothb, y = ~both_trendb,
data = smooth_frame_bothb,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothb,
'</br> Median Log Copies: ', round(both_trendb, digits = 2)),
line = list(color = '#D95F02', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothb, ymin = ~both_yminb, ymax = ~both_ymaxb,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothb, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxb, digits = 2),
'</br> Min Log Copies: ', round(both_yminb, digits = 2)),
name = "",
fillcolor = '#D95F02',
line = list(color = '#D95F02')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF B") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfb_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#D95F02', size = 6, opacity = 0.65))
p_wrf_b
save(p_wrf_b, file = "./site_objects/wrf_b_year2.rda")
#**************************************WRF C PLOT********************************************** #add trendlines #extract data from geom_smooth # *********************************span 0.6*********************************** #*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_bothc <- ggplot(wrfc_both, aes(x = date, y = log_total_copies_both)) +
stat_smooth(aes(outfit=fit_bothc<<-..y..), method = "loess", color = '#E7298A',
span = 0.3, n = 127)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothc
## `geom_smooth()` using formula 'y ~ x'
fit_bothc
## [1] 10.59277 10.71564 10.83236 10.94311 11.04804 11.14730 11.24105 11.32978
## [9] 11.41356 11.49197 11.56457 11.63094 11.69064 11.73940 11.77553 11.80243
## [17] 11.82352 11.84221 11.86193 11.88608 11.91372 11.94102 11.96703 11.99079
## [25] 12.01136 12.02780 12.03915 12.04551 12.04960 12.05434 12.06266 12.07751
## [33] 12.10182 12.13852 12.19103 12.25626 12.32741 12.39765 12.46017 12.50818
## [41] 12.53484 12.51680 12.49606 12.49710 12.48670 12.46963 12.45063 12.43445
## [49] 12.42585 12.42956 12.45190 12.49170 12.54252 12.59793 12.65149 12.69677
## [57] 12.72731 12.74308 12.74979 12.74959 12.74464 12.73711 12.72917 12.72296
## [65] 12.71886 12.71518 12.71101 12.70545 12.69761 12.68656 12.67142 12.64922
## [73] 12.61982 12.58657 12.55280 12.52184 12.49144 12.45833 12.42435 12.39132
## [81] 12.36105 12.33538 12.31613 12.30249 12.29198 12.28393 12.27764 12.27242
## [89] 12.26760 12.26247 12.26178 12.26765 12.27550 12.28078 12.27890 12.26530
## [97] 12.23540 12.18469 12.11694 12.04001 11.96178 11.89012 11.83291 11.79801
## [105] 11.76128 11.72993 11.73424 11.76289 11.80852 11.86374 11.92117 11.97345
## [113] 12.01318 12.04311 12.07032 12.09398 12.11326 12.12731 12.13530 12.13639
## [121] 12.13125 12.12107 12.10580 12.08536 12.05971 12.02878 11.99252
#assign fits to a vector
both_trendc <- fit_bothc
#extract y min and max for each
limits_bothc <- ggplot_build(extract_bothc)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothc <- as.data.frame(limits_bothc)
both_yminc <- limits_bothc$ymin
both_ymaxc <- limits_bothc$ymax
#reassign dataframes (just to be safe)
work_bothc <- wrfc_both
#fill in missing dates to smooth fits
work_bothc <- work_bothc %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothc <- work_bothc$date
#create a new smooth dataframe to layer
smooth_frame_bothc <- data.frame(date_vec_bothc, both_trendc, both_yminc, both_ymaxc)
#WRF C
#plot smooth frames
p_wrf_c <- plotly::plot_ly() %>%
plotly::add_lines(x = ~date_vec_bothc, y = ~both_trendc,
data = smooth_frame_bothc,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothc,
'</br> Median Log Copies: ', round(both_trendc, digits = 2)),
line = list(color = '#E7298A', size = 8, opacity = 0.65),
showlegend = FALSE) %>%
layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothc, ymin = ~both_yminc, ymax = ~both_ymaxc,
showlegend = FALSE,
opacity = 0.25,
hoverinfo = "text",
text = ~paste('</br> Date: ', date_vec_bothc, #leaving in case we want to change
'</br> Max Log Copies: ', round(both_ymaxc, digits = 2),
'</br> Min Log Copies: ', round(both_yminc, digits = 2)),
name = "",
fillcolor = '#E7298A',
line = list(color = '#E7298A')) %>%
layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies",
showline = TRUE,
automargin = TRUE)) %>%
layout(xaxis = list(title = "Date")) %>%
layout(title = "WRF C") %>%
plotly::add_markers(x = ~date, y = ~log_total_copies_both,
data = wrfc_both,
hoverinfo = "text",
showlegend = FALSE,
text = ~paste('</br> Date: ', date,
'</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
marker = list(color = '#E7298A', size = 6, opacity = 0.65))
p_wrf_c
save(p_wrf_c, file = "./site_objects/wrf_c_year2.rda")
keeping in case
#save(wrfa_both, file = "./plotly_objs/wrfa_both.rda")
#save(wrfb_both, file = "./plotly_objs/wrfb_both.rda")
#save(wrfc_both, file = "./plotly_objs/wrfc_both.rda")
#save(date_vec_botha, file = "./plotly_objs/date_vec_botha.rda")
#save(date_vec_bothb, file = "./plotly_objs/date_vec_bothb.rda")
#save(date_vec_bothc, file = "./plotly_objs/date_vec_bothc.rda")
#save(both_ymina, file = "./plotly_objs/both_ymina.rda")
#save(both_ymaxa, file = "./plotly_objs/both_ymaxa.rda")
#save(both_yminb, file = "./plotly_objs/both_yminb.rda")
#save(both_ymaxb, file = "./plotly_objs/both_ymaxb.rda")
#save(both_yminc, file = "./plotly_objs/both_yminc.rda")
#save(both_ymaxc, file = "./plotly_objs/both_ymaxc.rda")